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菌落形态学
Bacterial Colony Morphology
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关注菌落的形态、大小、边缘、突起、颜色、质地等等，都是区分细菌的重要手段。
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Purpose Identifying and categorizing different, isolated bacterial colonies
based upon varied appearance and morphology (form and structure) will
permit the selection and transfer of different species from a mixed culture,
and allow transfer of a single colony to a sterile medium for cultivation
of a pure culture. This exercise also shows how many diverse bacteria
and fungi are present in our environment, as exhibited by the varied morphologies.
Principles When a single bacterial cells is deposited on the surface of
a nutritive medium, it begins to divide exponentially. After thousands
(up to billions) of cells are formed, a visable mass appears. This mass
of cells is called a colony. Each species of bacterial or fungal organism
will exhibit characteristic colonies.
Additional Information Colony charachterisitcs are described in standard
terminology to permit interpretation by other persons. These terms may
appear to be complicated; however, you will soon become familiar with
them. Remember: the best descriptions are complete, but concise.
Terms
1. Colony shape and size: round, irregular, punctiform (tiny)
2. Margin (edge): entire (smooth), undulate (wavy), lobate (lobed)
3. Elevation: convex, umbonate, flat, raised
4. Color: color or pigment, plus opaque, translucent, shiny or dull
5. Texture: moist, mucoid, dry (or rough).
Use the images below to practice describing different colony morphologies.