内切酶活性表_生物化学与分子生物学_生物名词库_食品专题

Enzyme		Supplied NEBuffer		% Activity in NEBuffers
				1		2		3		4
Aat II		4		0		50		50		100
Acc I		4		50		50		10		100
Acc65 I		3 BSA		10		75		100		25
Aci I		3		25		50		100		50
Acl I		4 BSA		10		10		0		100
Afe I *		SE-Y		25		50		25		100
Afl II		2 BSA		50		100		25		100
Afl III		3 BSA		25		75		100		50
Age I		1		100		50		10		75
Ahd I		4 BSA		25		75		0		100
Ale I		4		10		20		10		100
Alu I		2		100		100		75		100
Alw I		4		50		100		10		100
AlwN I		4		50		100		50		100
Apa I @25°C		4 BSA		25		50		0		100
ApaL I		4 BSA		100		100		10		100
Apo I @50°C		3 BSA		10		75		100		75
Asc I		4		0		10		10		100
Ase I		3		NR		75		^dd100		NR
AsiS I		3 BSA		50		100		100		50
Ava I		4		10		75		10		100
Ava II		4		50		75		10		100
Avr II		2		100		100		50		100
Bae I @25°C		2 BSA SAM		50		100		50		75
BamH I		U^dd BSA		75		100		50		75
Ban I		4		50		100		50		100
Ban II		4		100		10		50		100
Bbs I		2		100		100		25		75
Bbv I		2		100		100		25		75
BbvC I		4		50		100		10		100
BceA I		U^dd BSA		25		NR		NR		75
Bcg I		U SAM		50		75		100		50
BciV I		4		100		25		10		100
Bcl I @50°C		3		50		100		^dd100		75
Bfa I		4		75		50		10		100
BfrB I		4 BSA		10		25		10		100
BfuA I @50°C		3		0		75		100		10
BfuC I		4 BSA		100		50		25		100
Bgl I		3		50		75		100		50
Bgl II		3		10		75		100		10
Blp I		4		50		100		10		100
Bme1580 I		2		75		100		75		75
BmgB I		3 BSA		0		25		100		10
Bmr I		2		75		100		75		100
Bmt I *		SE-W		25		100		10		50
Bpm I		3 BSA		75		100		100		100
Bpu10 I*		SE-K		0		25		100		25
BpuE I @25°C		2 SAM		75		100		10		75
Bsa I @50°C		3		75		75		100		100
BsaA I		3		75		75		100		75
BsaB I @60°C		2		75		100		100		100
BsaH I		4 BSA		50		100		100		100
BsaJ I @60°C		2		100		100		100		100
BsaW I @60°C		2 BSA		50		100		50		50
BsaX I		4		75		100		10		100
BseR I		2		100		100		75		100
BseY I		3		10		50		100		50
Bsg I		4 SAM		50		75		50		100
BsiE I @60°C		2 BSA		50		100		10		100
BsiHKA I @65°C		3 BSA		50		100		100		100
BsiW I @55°C		3		100		100		100		25
Bsl I @55°C		3		10		50		100		75
Bsm I @65°C		2		75		100		75		75
BsmA I @55°C		3		50		100		100		50
BsmB I @55°C		3		75		100		100		100
BsmF I @65°C		4 BSA		25		50		50		100
BsoB I @65°C		2		10		100		100		50
Bsp1286 I		4 BSA		25		25		25		100
BspCN I @25°C		1 BSA SAM		100		75		10		75
BspD I		4		25		75		50		100
BspE I		3		0		10		100		0
BspH I		4		10		100		50		100
BspM I		3		NR		NR		100		NR
Bsr I @65°C		3		0		50		100		10
BsrB I		2		50		100		100		100
BsrD I @60°C		2 BSA		50		100		50		75
BsrF I		U		25		100		75		75
BsrG I		2 BSA		25		100		10		100
BssH II @50°C		3		100		100		^dd100		100
BssK I @60°C		3 BSA		0		50		100		50
BssS I		3		0		50		^dd100		10
BstAP I * @60°C		SE-W		25		25		100		25
BstB I @65°C		4		75		50		25		100
BstE II @60°C		3		50		75		100		75
BstF5 I * @65°C		SE-Y		75		50		25		100
BstN I @60°C		2 BSA		10		100		100		75
BstU I @60°C		2		100		100		50		100
BstX I @55°C		3		25		100		100		50
BstY I @60°C		2		50		100		75		100
BstZ17 I		3		NR		NR		100		NR
Bsu36 I		3 BSA		0		25		100		0
Btg I		3 BSA		25		50		100		100
Bts II		4 BSA		100		50		50		100
Cac8 I		3		50		75		100		75
Cla I		4 BSA		10		50		50		100
Dde I		3		75		100		100		75
Dpn I		4		100		100		75		100
Dpn II		U		NR		NR		^dd100		NR
Dra I		4		75		75		50		100
Dra III		3 BSA		100		75		100		25
Drd I		4		25		50		10		100
Eae I		1		100		100		50		100
Eag I		3		10		25		100		10
Ear I		1		100		100		50		100
Eci I		2 BSA		50		100		100		50
EcoN I		4		100		100		75		100
EcoO109 I		4 BSA		100		100		75		100
EcoR I		U^dd		100		100		100		100
EcoR V		3 BSA		50		75		100		50
Fat I *		SE-G		10		100		50		50
Fau I * @55°C		SE-B		100		25		10		50
Fnu4H I		4		10		25		25		100
Fok I		4		50		100		75		100
Fse I		4 BSA		100		75		0		100
Fsp I		4		10		75		10		100
Hae II		4 BSA		75		100		50		100
Hae III		2		50		100		25		75
Hga I		1		100		75		50		100
Hha I		4 BSA		75		100		100		100
Hinc II		3 BSA		75		100		100		100
Hind III		2		50		100		10		50
Hinf I		2		75		100		75		75
HinP1 I		2		100		100		100		100
Hpa I		4		25		50		10		100
Hpa II		1		100		50		10		100
Hph I		4		NR		75		0		100
Hpy99 I		4 BSA		10		10		0		100
Hpy188 I		4		50		25		10		100
Hpy188 III		4 BSA		100		100		10		100
HpyCH4III		4		100		50		25		100
HpyCH4IV		1		100		25		10		25
HpyCH4V		4		50		50		25		100
I-Ceu I		4 BSA		10		10		0		100
I-Sce I		U BSA		10		50		50		50
Kas I		2 BSA		75		100		75		75
Kpn I		1 BSA		100		75		0		50
	Mbo I		3		75		100		100		100
	Mbo II		2		100		100		50		100
	McrBC		2 BSA GTP		50		100		10		75
	Mfe I		4		75		50		10		100
	Mlu I		3		25		75		100		50
	Mly I		4 BSA		50		50		25		100
	Mme I		4 SAM		NR		NR		NR		100
	Mnl I		2 BSA		75		100		50		75
	Msc I		4		75		75		75		100
	Mse I		2 BSA		75		100		75		75
	Msl I		2		100		100		25		100
	Msp I		2		75		100		50		50
	MspA1 I		4 BSA		50		100		50		100
	Mwo I @60°C		U		10		75		100		75
	Nae I		1		100		75		10		75
	N.Alw I		2		25		100		25		50
	Nar I		1		100		75		75		75
	N.BbvC IA		2		50		100		10		100
	N.BbvC IB		2		50		100		10		100
	N.BstNB I @55°C		U		0		10		10		10
	Nci I		4		100		25		10		100
	Nco I		4		100		100		100		100
	Nde I		4		75		100		75		100
	NgoM IV		4		100		50		10		100
	Nhe I		2 BSA		100		100		10		100
	Nla III		4 BSA		25		25		25		100
	Nla IV		4 BSA		10		10		10		100
	Not I		3 BSA		0		75		100		50
	Nru I		U		0		10		75		10
	Nsi I		U		25		25		50		10
	Nsp I		2 BSA		100		100		0		100
	Pac I		1 BSA		100		75		10		100
	PaeR7 I		4		25		100		10		100
	Pci I *		SE-O		25		75		100		50
	PflF I		4 BSA		0		25		25		100
	PflM I		3 BSA		0		100		100		50
	PI-Psp I @65°C		U BSA		0		10		10		10
	PI-Sce I		U BSA		0		0		0		0
	Ple I		4		100		100		75		100
	Pme I		4 BSA		0		50		10		100
	Pml I		1 BSA		100		75		0		75
	PpuM I		4		0		25		0		100
	PshA I		4 BSA		50		75		10		100
	Psi I *		SE-B		50		100		0		25
	PspG I @75°C		2		75		100		50		100
	PspOM I *		SE-Y		25		100		50		100
	Pst I		3 BSA		75		75		100		50
	Pvu I		3 BSA		10		75		100		10
	Pvu II		2		100		100		100		100
	Rsa I		1		100		100		50		100
	Rsr II		4		25		75		10		100
	Sac I		1 BSA		100		50		10		100
	Sac II		4		25		75		10		100
	Sal I		U BSA		0		0		50		0
	Sap I		4		75		50		0		100
	Sau3A I		U BSA		100		50		10		100
	Sau96 I		4		50		100		100		100
	Sbf I *		SE-Y		75		50		0		100
	Sca I		U		25		75		^dd50		25
	ScrF I		4		100		100		100		100
	SexA I		3 BSA		25		25		100		100
	SfaN I		3		0		75		100		25
	Sfc I		4 BSA		75		50		10		100
	Sfi I @50°C		2 BSA		0		100		10		100
	Sfo I		2		25		100		50		50
	SgrA I		4		100		50		10		100
	Sma I @25°C		4		0		0		0		100
	Sml I @55°C		4 BSA		25		75		25		100
	SnaB I		4 BSA		25		50		25		^dd100
	Spe I		2 BSA		75		100		25		75
	Sph I		2		100		100		50		100
	Ssp I		U		50		100		50		50
	Stu I		2		100		100		50		100
	Sty I		3 BSA		25		75		100		10
	StyD4 I		2		10		100		100		100
	Swa I @25°C		3 BSA		10		10		100		10
	Taq I @65°C		U BSA		50		75		75		75
	Tfi I @65°C		3		100		100		^dd100		100
	Tli I @75°C		3 BSA		25		25		100		10
	Tse I @65°C		3		75		100		100		75
	Tsp45 I @65°C		1 BSA		100		25		0		75
	Tsp509 I @65°C		1		100		100		100		NR
	TspR I @65°C		4 BSA		25		50		25		100
	Tth111 I @65°C		1		100		25		25		100
	Xba I		2 BSA		0		100		75		75
	Xcm I		2		10		100		50		50
	Xho I		2 BSA		75		100		100		100
	Xma I		4 BSA		25		50		0		100
	Xmn I		2 BSA		100		100		75		100
	Zra I *		SE-B		100		100		25		100

• 基因突变的检测方法	• PCR技术基本原理
• 生化实验室常用技术参数资料	• PCR反应特点
• PCR污染与对策	• 实时定量PCR应用中的问题
• 实时定量PCR实验方案优化	• PCR常见问题
• 原位杂交（以斑马鱼为例）	• 实时定量PCR荧光材料新进展